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Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters

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Extracellular Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Elucidating extracellular vesicle (EV; e.g., exosomes, microvesicles) delivery and translation of its RNA cargo with an accurate spatiotemporal resolution is critical in helping understand EV’s role under normal and pathological conditions. We here describe a multiplexed fluorescent and bioluminescent reporter strategy to simultaneously monitor and quantify EV delivery, as well as EV-RNA translation in EV-recipient cells.

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Acknowledgment

This work was supported by the Ministry of Science and Technology of Taiwan (MOST), Taiwan (MOST 104-2320-B-007-005-MY2, C.P.L), and United States NIH grants CA069246 (Xandra O. Breakefield, Bakhos A. Tannous, and Ralph Weissleder), U19CA179563 (X.O.B and Thorsten R. Mempel), Voices Against Brain Cancer (X.O.B. and C.P.L.), and the Canadian Institutes of Health Research (CIHR; C.P.L.).

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Authors and Affiliations

  1. Institute of Biomedical Engineering, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan

    Anthony Yan-Tang Wu & Charles Pin-Kuang Lai

  2. Institute of Atomic and Molecular Sciences, Academia Sinica, No. 1, Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan

    Anthony Yan-Tang Wu & Charles Pin-Kuang Lai

Authors
  1. Anthony Yan-Tang Wu
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  2. Charles Pin-Kuang Lai
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Corresponding author

Correspondence to Charles Pin-Kuang Lai .

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Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Wu, A.YT., Lai, C.PK. (2017). Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters. In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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