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Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)

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Book cover Extracellular Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1660))

Abstract

Extracellular vesicles (EVs) have recently attracted substantial attention due to the potential diagnostic and therapeutic relevance. Although a variety of techniques have been used to isolate and analyze EVs, it is still far away from satisfaction. Size-exclusion chromatography (SEC), which separates subjects by size, has been widely applied in protein purification and analysis. The purpose of this chapter is to show the applications of size-exclusion high-performance liquid chromatography (HPLC) as methods for EV characterization of impurities or contaminants of small size, and thus for quality assay for the purity of the samples of EVs.

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Author information

Authors and Affiliations

  1. Department of Radiology and Medical Imaging, University of Virginia, PO BOX 801339, Fontaine Research Park, Rm180, 480 Ray C Hunt Drive, Charlottesville, 22903, VA, USA

    Tao Huang Ph.D

  2. Department of Radiology and Medical Imaging, University of Virginia, PO BOX 801339, Fontaine Research Park, Rm. 282, 480 Ray C. Hunt Drive, Charlottesville, VA, 22903, USA

    Jiang He Ph.D

Authors
  1. Tao Huang Ph.D
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  2. Jiang He Ph.D
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Corresponding author

Correspondence to Jiang He Ph.D .

Editor information

Editors and Affiliations

  1. CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  2. Predicine, Inc., Hayward, California, USA

    Shidong Jia

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Huang, T., He, J. (2017). Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC). In: Kuo, W., Jia, S. (eds) Extracellular Vesicles. Methods in Molecular Biology, vol 1660. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7253-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7253-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7251-7

  • Online ISBN: 978-1-4939-7253-1

  • eBook Packages: Springer Protocols

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