Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)

  • Tao Huang
  • Jiang HeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1660)


Extracellular vesicles (EVs) have recently attracted substantial attention due to the potential diagnostic and therapeutic relevance. Although a variety of techniques have been used to isolate and analyze EVs, it is still far away from satisfaction. Size-exclusion chromatography (SEC), which separates subjects by size, has been widely applied in protein purification and analysis. The purpose of this chapter is to show the applications of size-exclusion high-performance liquid chromatography (HPLC) as methods for EV characterization of impurities or contaminants of small size, and thus for quality assay for the purity of the samples of EVs.

Key words

Size-exclusion chromatography High-performance liquid chromatography (HPLC) Extracellular vesicles Exosomes 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Radiology and Medical ImagingUniversity of VirginiaCharlottesvilleUSA
  2. 2.Department of Radiology and Medical ImagingUniversity of VirginiaCharlottesvilleUSA

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