Abstract
A simple point-inoculation method using Novec™ 7100, a volatile engineered fluid, is presented for the assessment of aggressiveness of Puccinia striiformis isolates on seedlings of wheat. The method allows for quantification of the applied inoculum with a minimal risk of cross-contamination of rust from leaves grown side by side. The method is also applicable for the assessment of qualitative differences inferred by compatible and incompatible host–pathogen interactions, and it can be adjusted to other cereal rust and powdery mildew fungi on other host species, and other plant growth stages as appropriate.
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Acknowledgments
This method was developed under the RUSTFIGHT (Grant number: 11-116241) funded by Innovation Fund Denmark, The Ministry of Higher Education and Science, Denmark, and GUDP, Ministry of Environment and Food of Denmark (grant no. 34120902567). The authors thank Steen Meier, Ellen Jørgensen, and Janne Hansen for technical support, and Annemarie Fejer Justesen, Mehran Patpour, and Julian Rodriguez-Algaba for valuable discussions.
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Sørensen, C.K., Thach, T., Hovmøller, M.S. (2017). Assessment of Aggressiveness of Puccinia striiformis on Wheat. In: Periyannan, S. (eds) Wheat Rust Diseases. Methods in Molecular Biology, vol 1659. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7249-4_4
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DOI: https://doi.org/10.1007/978-1-4939-7249-4_4
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