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Analysis of Prion Protein Structure Using Nuclear Magnetic Resonance Spectroscopy

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Prions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1658))

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a powerful experimental tool for obtaining information on three-dimensional (3D) structures of proteins at atomic resolution. In inherited forms of prion diseases, misfolding of cellular prion protein, PrPC, into its pathological form, PrPSc, is caused by mutations in the human prion protein gene (PRNP). Understanding of the earliest stages of the conformational changes leading to spontaneous generation of prions in inherited forms of prion diseases may benefit from detailed structural analysis of different human (Hu) PrP variants. Here, we describe the protocol for structure determination of HuPrP variants by NMR spectroscopy in solution that consists of preparation of NMR samples, acquisition of NMR data, NMR resonance assignments, and structure calculation.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102A, 10000, Zagreb, Croatia

    Ivana Biljan

  2. Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, 1000, Ljubljana, Slovenia

    Gregor Ilc & Janez Plavec

  3. EN-FIST Center of Excellence, Trg OF 13, 1000, Ljubljana, Slovenia

    Gregor Ilc & Janez Plavec

  4. Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000, Ljubljana, Slovenia

    Janez Plavec

Authors
  1. Ivana Biljan
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  2. Gregor Ilc
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  3. Janez Plavec
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Corresponding author

Correspondence to Janez Plavec .

Editor information

Editors and Affiliations

  1. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia

    Victoria A. Lawson

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Biljan, I., Ilc, G., Plavec, J. (2017). Analysis of Prion Protein Structure Using Nuclear Magnetic Resonance Spectroscopy. In: Lawson, V. (eds) Prions. Methods in Molecular Biology, vol 1658. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7244-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7244-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7242-5

  • Online ISBN: 978-1-4939-7244-9

  • eBook Packages: Springer Protocols

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