Abstract
Bacteria in the biofilm mode of growth cause numerous problematic infections due to their resistance to antimicrobials and the immune system. Because conventional antimicrobial compounds cannot efficiently eradicate biofilm infections, we urgently need new efficient anti-biofilm drugs. The secondary messenger c-di-GMP is a positive regulator of biofilm formation in many clinically relevant bacteria, and it is assumed that drugs that lower the intracellular level of c-di-GMP will force biofilm bacteria into a more treatable planktonic lifestyle. We describe a protocol for high-throughput screening of chemical libraries for compounds that lower the c-di-GMP level in bacteria, and potentially can serve as lead compounds in the development of novel biofilm dismantling drugs.
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Acknowledgments
This work was supported by grants from and the Danish Council for Independent Research (DFF–1323-00177) to TTN, and from the Danish Strategic Research Council and the Lundbeck Foundation (R198-2015-486) to MG.
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Groizeleau, J., Andersen, J.B., Givskov, M., Berthelsen, J., Tolker-Nielsen, T. (2017). High-Throughput Screening for Compounds that Modulate the Cellular c-di-GMP Level in Bacteria. In: Sauer, K. (eds) c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7240-1_33
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DOI: https://doi.org/10.1007/978-1-4939-7240-1_33
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7239-5
Online ISBN: 978-1-4939-7240-1
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