RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity
Pathogen recognition receptors (PRR)s and their cognate pathogen-associated molecular pattern (PAMP) represent the basis of innate immune activation and immune response induction driven by the host-pathogen interaction that occurs during microbial infection in humans and other animals. For RNA virus infection such as hepatitis C virus (HCV) and others, specific motifs within viral RNA mark it as nonself and visible to the host as a PAMP through interaction with RIG-I-like receptors including retinoic inducible gene-I (RIG-I). Here, we present methods for producing and using HCV PAMP RNA as a molecular tool to study RIG-I and its signaling pathway, both in vitro and in vivo, in innate immune regulation.
Key wordsRNA PAMP Innate immunity Rig-I Pathogen recognition receptor
- 18.Kell A, Stoddard M, Li H, Marcotrigiano J, Shaw GM, Gale M Jr (2015) Pathogen-associated molecular pattern recognition of hepatitis C virus transmitted/founder variants by RIG-I is dependent on U-Core length. J Virol 89(21):11056–11068. doi: 10.1128/JVI.01964-15CrossRefPubMedPubMedCentralGoogle Scholar
- 25.Sumpter R Jr, Loo YM, Foy E, Li K, Yoneyama M, Fujita T, Lemon SM, Gale M Jr (2005) Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I. J Virol 79(5):2689–2699. doi: 10.1128/JVI.79.5.2689-2699.2005CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Loo YM, Owen DM, Li K, Erickson AK, Johnson CL, Fish PM, Carney DS, Wang T, Ishida H, Yoneyama M, Fujita T, Saito T, Lee WM, Hagedorn CH, Lau DT, Weinman SA, Lemon SM, Gale M Jr (2006) Viral and therapeutic control of IFN-beta promoter stimulator 1 during hepatitis C virus infection. Proc Natl Acad Sci U S A 103(15):6001–6006. doi: 10.1073/pnas.0601523103CrossRefPubMedPubMedCentralGoogle Scholar