Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods

  • Sarah J. Poynter
  • Stephanie J. DeWitte-OrrEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1656)


Long double-stranded (ds) RNA molecules are produced as a byproduct of viral replication. Studying virus-derived dsRNA is important for understanding virus replication, understanding host responses to virus infections, and as a diagnostic tool for virus presence and replication. Here, we describe four different techniques for visualizing dsRNA; two antibody-dependent methods (immunoblotting and immunocytochemistry), as well as two antibody-independent methods (differential digestion and acridine orange staining). The benefits and disadvantages of each technique are also discussed.

Key words

dsRNA Immunoblot Differential digestion Viral dsRNA Acridine orange Immunocytochemistry 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of BiologyWilfrid Laurier UniversityWaterlooCanada
  2. 2.Departments of Health Sciences and BiologyWilfrid Laurier UniversityWaterlooCanada

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