Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes

  • Tianyun Long
  • Rui LuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1656)


Northern blot analysis has been widely used as a tool for detection and characterization of specific RNA molecules. When coupled with radioactive probe northern blot allows for robust detection and characterization of small RNA molecules of trace amount. Here, we describe the detection and size characterization of virus-derived small interfering RNAs (vsiRNAs) in C. elegans using nonradioactive DNA oligo probes in northern blotting. Our protocol allows for the detection and characterization of not only primary vsiRNAs but also secondary vsiRNAs, a class of single-stranded vsiRNAs that has distinct migration pattern, and can be easily adapted to the detection of vsiRNAs in other organisms.

Key words

Northern blot Nonradioactive Virus-derived siRNA DIG-labeled probe PAGE gel electrophoresis Caenorhabditis elegans 



This work was supported by NIH grant R01GM119012-01A1 to R.L.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Biological SciencesLouisiana State UniversityBaton RougeUSA

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