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Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs

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Book cover Innate Antiviral Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1656))

Abstract

The recently described group 2 innate lymphoid cells (ILC2) exert critical roles in type 2 immune responses, epithelial repair at mucosal tissues and metabolic homeostasis. ILC2 release large amounts of type 2 cytokines such as interleukin 4 (IL-4), IL-5, and IL-13, driving type 2 immunity such as the defense against helminths. However, if not tightly regulated ILC2 can trigger unwanted type 2 immunopathologies including allergic airway inflammation, airway hyper-responsiveness, and atopic dermatitis. Viral respiratory tract infections, archetypal triggers of type 1 immune responses, often give rise to pulmonary type 2 immunopathologies such as asthma and asthma exacerbations. Interestingly, pulmonary viral infections induce the release of IL-33, followed by induction of ILC2-mediated pulmonary type 2 immunopathology independent of the adaptive immune system. Due to their scarcity at steady state but also after infection and inflammation, pulmonary ILC2 are challenging to work with. In this chapter, we describe the detection and isolation procedure of pulmonary mouse ILC2 by flow cytometry and compare four distinct enzymatic mouse lung tissue processing protocols for optimized cell yield.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, McGill University Research Centre on Complex Traits (MRCCT), McGill University, 3649 Promenade-Sir-William Osler, Bellini Pavilion Room 371, Montreal, QC, Canada, H3G 0B1

    Claudia U. Duerr & Jörg H. Fritz

  2. Department of Physiology, McGill University Research Centre on Complex Traits (MRCCT), McGill University, 3649 Promenade-Sir-William Osler, Bellini Pavilion Room 371, Montreal, QC, Canada, H3G 0B1

    Jörg H. Fritz

Authors
  1. Claudia U. Duerr
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  2. Jörg H. Fritz
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Corresponding author

Correspondence to Jörg H. Fritz .

Editor information

Editors and Affiliations

  1. Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada

    Karen Mossman

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Duerr, C.U., Fritz, J.H. (2017). Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs. In: Mossman, K. (eds) Innate Antiviral Immunity. Methods in Molecular Biology, vol 1656. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7237-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7237-1_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7236-4

  • Online ISBN: 978-1-4939-7237-1

  • eBook Packages: Springer Protocols

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