Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma

  • Ananda Ayyappan Jaguva Vasudevan
  • Wolfgang Goering
  • Dieter Häussinger
  • Carsten MünkEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)


Members of the APOBEC3 (A3) family of enzymes were shown to act in an oncogenic manner in several cancer types. Immunodetection of APOBEC3A (A3A), APOBEC3B (A3B), and APOBEC3G (A3G) proteins is particularly challenging due to the large sequence homology of these proteins and limited availability of antibodies. Here we combine independent immunoblotting with an in vitro activity assay technique, to detect and categorize specific A3s expressed in urothelial bladder cancer and other cancer cells.

Key words

APOBEC3 Cytidine deaminase Urothelial bladder cancer Mutation Deamination assay Cancer cell 



We would like to thank Klaus Strebel and the NIH AIDS Research and Reference Reagent Program for anti-ApoC17 (A3G) antibody. We are grateful to W. A. Schulz for his constant support. CM is supported by the Heinz Ansmann foundation.


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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Ananda Ayyappan Jaguva Vasudevan
    • 1
    • 2
  • Wolfgang Goering
    • 2
    • 3
  • Dieter Häussinger
    • 1
  • Carsten Münk
    • 1
    Email author
  1. 1.Clinic for Gastroenterology, Hepatology, and Infectiology, Medical FacultyHeinrich Heine University DüsseldorfDüsseldorfGermany
  2. 2.Department of Urology, Medical FacultyHeinrich Heine University DüsseldorfDüsseldorfGermany
  3. 3.Institute of Pathology, Medical FacultyHeinrich Heine University DüsseldorfDüsseldorfGermany

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