Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma
Members of the APOBEC3 (A3) family of enzymes were shown to act in an oncogenic manner in several cancer types. Immunodetection of APOBEC3A (A3A), APOBEC3B (A3B), and APOBEC3G (A3G) proteins is particularly challenging due to the large sequence homology of these proteins and limited availability of antibodies. Here we combine independent immunoblotting with an in vitro activity assay technique, to detect and categorize specific A3s expressed in urothelial bladder cancer and other cancer cells.
Key wordsAPOBEC3 Cytidine deaminase Urothelial bladder cancer Mutation Deamination assay Cancer cell
We would like to thank Klaus Strebel and the NIH AIDS Research and Reference Reagent Program for anti-ApoC17 (A3G) antibody. We are grateful to W. A. Schulz for his constant support. CM is supported by the Heinz Ansmann foundation.
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