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Algorithm for the Automated Evaluation of NAT2 Genotypes

  • Georg MichaelEmail author
  • Ricarda Thier
  • Meinolf Blaszkewicz
  • Silvia Selinski
  • Klaus Golka
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)

Abstract

N-Acetyltransferase 2 (NAT2) genotyping by PCR and RFLP-based methods provides information on seven single nucleotide polymorphisms (SNPs) without deriving the chromosomal phase (haplotype). So genotyping results must be processed to get all possible NAT2 haplotype (or allele) combinations. Here we describe the procedure for genotyping and present a program based on Microsoft® Access® which automatically generates all possible haplotype pairs for a given unphased NAT2 genotype. NAT2 haplotypes are important to predict the NAT2 phenotype.

Key words

N-Acetyltransferase 2 Alleles Automated evaluation Computer program Genotyping Haplotype 

Supplementary material

394598_1_En_7_MOESM1_ESM.docx (17 kb)
Source Code_NAT2Genotyping (DOCX 17 kb)

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Georg Michael
    • 1
    Email author
  • Ricarda Thier
    • 2
    • 3
  • Meinolf Blaszkewicz
    • 2
  • Silvia Selinski
    • 2
  • Klaus Golka
    • 2
  1. 1.SteinenGermany
  2. 2.Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo)DortmundGermany
  3. 3.School of MedicineGriffith UniversitySouthportAustralia

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