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Immunohistochemical Analysis of Urothelial Carcinoma Tissues for Proliferation and Differentiation Markers

  • Michael Rose
  • Nadine T. GaisaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)

Abstract

Immunohistochemistry is a standard method in histopathology and enables the localized detection of proteins in histological tissue sections. Specific antibodies are bound to cellular antigens, captured by secondary antibodies or polymers, linked to enzymes and visualized by chromogenic substrates. Here, we describe an automated staining technique for larger slide batches as well as a manual protocol for only few slides using a polymer technique. We focus on differentiation markers, measures of cell proliferation, and therapeutic targets in benign and malignant urothelial tissues.

Key words

Immunohistochemistry Polymer (Cyto)keratins Uroplakins Ki67 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Institute of PathologyRWTH Aachen UniversityAachenGermany

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