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Visualization and Quantitative Measurement of Drug-Induced Platinum Adducts in the Nuclear DNA of Individual Cells by an Immuno-Cytological Assay

  • Margarita Melnikova
  • Jürgen ThomaleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)

Abstract

Immunocytological staining with adduct-specific antibodies allows the visualization and measurement of structurally defined types of DNA damage in the nuclei of individual cells. Here we describe an immunocytological assay (ICA) procedure for the localization and quantification of such damage, in particular induced by platinum-based anticancer drugs, in cell lines, in primary cell suspensions and in frozen tissue sections.

Key words

DNA adducts Cisplatin Monoclonal antibody Immunofluorescence Single cell analysis Quantitative image analysis 

Notes

Acknowledgement

We thank Maria Eynck (IFZ) for excellent technical assistance.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Institute of Cell Biology (Cancer Research)University of Duisburg-Essen Medical SchoolEssenGermany

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