Visualization and Quantitative Measurement of Drug-Induced Platinum Adducts in the Nuclear DNA of Individual Cells by an Immuno-Cytological Assay
Immunocytological staining with adduct-specific antibodies allows the visualization and measurement of structurally defined types of DNA damage in the nuclei of individual cells. Here we describe an immunocytological assay (ICA) procedure for the localization and quantification of such damage, in particular induced by platinum-based anticancer drugs, in cell lines, in primary cell suspensions and in frozen tissue sections.
Key wordsDNA adducts Cisplatin Monoclonal antibody Immunofluorescence Single cell analysis Quantitative image analysis
We thank Maria Eynck (IFZ) for excellent technical assistance.
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