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Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum

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Urothelial Carcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1655))

Abstract

The epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases (RTK) consists of four members: EGFR1/ErbB1/HER1, ErbB2/HER2, ErbB3/HER3, and HER4/ErbB4. Signaling through these receptors regulates many key cellular activities, such as cell division, migration, adhesion, differentiation, and apoptosis. The ErbB family has been shown to be overexpressed in different types of cancers and is a target of several inhibitors already in clinical trials. ErbB3 lacks a functional tyrosine kinase domain and therefore has not been as extensively studied as the other members of this family, but its importance in activating downstream pathways, such as the PI3K/Akt pathway, makes this RTK a worthy investigation target, especially in urothelial carcinoma where the PI3K/Akt pathway is vital for progression. In recent times, ErbB3 overexpression has been linked to drug resistance and progression of various diseases, especially cancer. ErbB3 levels in the serum were shown in many cases to be reflective of its role in disease progression, and therefore detection of serum ErbB3 levels during treatment may be of importance.

Here we describe two methods for detecting ErbB3 protein in serum from patients who have undergone a clinical trial, utilizing two well-established methods in molecular biology—western blotting and ELISA, focusing on sample preparation and troubleshooting.

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Acknowledgement

This work was supported by Biomedical Laboratory Research & Development (BLRD) Merit Awards (I01BX000400, PMG; I01BX001784, CXP) from the Department of Veterans Affairs, and by the National Institutes of Health (Awards R01CA133209 and R01CA185509, PMG).

Author information

Authors and Affiliations

  1. VA Northern California Health Care System, University of California at Davis, Sacramento, CA, USA

    Leandro S. D’Abronzo, Chong-Xian Pan & Paramita M. Ghosh Ph.D.

  2. Department of Urology, University of California at Davis, Sacramento, CA, USA

    Leandro S. D’Abronzo, Chong-Xian Pan & Paramita M. Ghosh Ph.D.

  3. Division of Hematology and Oncology, Department of Internal Medicine, University of California at Davis, Sacramento, CA, USA

    Chong-Xian Pan

  4. Department of Biochemistry and Molecular Medicine, University of California at Davis, Sacramento, CA, USA

    Paramita M. Ghosh Ph.D.

  5. Department of Urology, University of California Davis School of Medicine, 4860 YStreet, Suite 3500, Sacramento, CA, 95817, USA

    Paramita M. Ghosh Ph.D.

Authors
  1. Leandro S. D’Abronzo
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  2. Chong-Xian Pan
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  3. Paramita M. Ghosh Ph.D.
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Corresponding author

Correspondence to Paramita M. Ghosh Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Urology, Heinrich Heine University, DĂĽsseldorf, Germany

    Wolfgang A Schulz

  2. Department of Urology, Heinrich Heine University, DĂĽsseldorf, Germany

    Michèle J. Hoffmann

  3. Department of Urology, Heinrich Heine University, DĂĽsseldorf, Germany

    GĂĽnter Niegisch

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D’Abronzo, L.S., Pan, CX., Ghosh, P.M. (2018). Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum. In: Schulz, W., Hoffmann, M., Niegisch, G. (eds) Urothelial Carcinoma. Methods in Molecular Biology, vol 1655. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7234-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7234-0_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7233-3

  • Online ISBN: 978-1-4939-7234-0

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