Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum

  • Leandro S. D’Abronzo
  • Chong-Xian Pan
  • Paramita M. GhoshEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)


The epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases (RTK) consists of four members: EGFR1/ErbB1/HER1, ErbB2/HER2, ErbB3/HER3, and HER4/ErbB4. Signaling through these receptors regulates many key cellular activities, such as cell division, migration, adhesion, differentiation, and apoptosis. The ErbB family has been shown to be overexpressed in different types of cancers and is a target of several inhibitors already in clinical trials. ErbB3 lacks a functional tyrosine kinase domain and therefore has not been as extensively studied as the other members of this family, but its importance in activating downstream pathways, such as the PI3K/Akt pathway, makes this RTK a worthy investigation target, especially in urothelial carcinoma where the PI3K/Akt pathway is vital for progression. In recent times, ErbB3 overexpression has been linked to drug resistance and progression of various diseases, especially cancer. ErbB3 levels in the serum were shown in many cases to be reflective of its role in disease progression, and therefore detection of serum ErbB3 levels during treatment may be of importance.

Here we describe two methods for detecting ErbB3 protein in serum from patients who have undergone a clinical trial, utilizing two well-established methods in molecular biology—western blotting and ELISA, focusing on sample preparation and troubleshooting.

Key words

EGFR ERBB3 Serum Urothelial carcinoma Western blot Elisa 



This work was supported by Biomedical Laboratory Research & Development (BLRD) Merit Awards (I01BX000400, PMG; I01BX001784, CXP) from the Department of Veterans Affairs, and by the National Institutes of Health (Awards R01CA133209 and R01CA185509, PMG).


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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Leandro S. D’Abronzo
    • 1
    • 2
  • Chong-Xian Pan
    • 1
    • 2
    • 3
  • Paramita M. Ghosh
    • 1
    • 2
    • 4
    • 5
    Email author
  1. 1.VA Northern California Health Care SystemUniversity of California at DavisSacramentoUSA
  2. 2.Department of UrologyUniversity of California at DavisSacramentoUSA
  3. 3.Division of Hematology and Oncology, Department of Internal MedicineUniversity of California at DavisSacramentoUSA
  4. 4.Department of Biochemistry and Molecular MedicineUniversity of California at DavisSacramentoUSA
  5. 5.Department of UrologyUniversity of California Davis School of MedicineSacramentoUSA

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