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Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin

  • Vladimir BobekEmail author
  • Katarina Kolostova
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)

Abstract

Monitoring of circulating tumor cells’ (CTCs) presence has the potential to improve therapeutic management of oncological diseases at an early stage and also to identify patients with increased risk of tumor progression or recurrence before the onset of clinically detected metastasis. Here we describe a new simplified efficient methodology for the separation and in vitro culturing of viable CTCs from peripheral blood by size-based filtration (MetaCell®). The isolation protocol yields preferentially cells bigger than 8 μm enabling further cytomorphological and molecular analysis.

Key words

CTCs Circulating tumor cells Prostate cancer Renal Bladder cancer Cultivation In vitro Gene expression 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Laboratory GeneticsUniversity Hospital Kralovske VinohradyPragueCzech Republic

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