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Quantitative RNA Analysis from Urine Using Real Time PCR

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Urothelial Carcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1655))

Abstract

Urine is emerging as a biological fluid suitable to perform liquid biopsy in a minimally invasive manner, a fundamental attribute for prevention and early detection of cancer. Urine biomarkers can be analyzed in voided urine, in urine sediment, and urine supernatant. In the case of urothelial carcinoma, in which tumor cells are in direct contact with urine, the assessment of the levels of biomarkers in the urinary cell fraction appears to be the most promising approach to identify diagnostic and prognostic biomarkers in a noninvasive way. Here, we describe a protocol to collect and process urine samples to obtain urinary exfoliated cells. Furthermore, we describe the methodology to isolate RNA from urinary cells and to quantify gene expression levels from these urinary cells.

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Authors and Affiliations

  1. Laboratory and Department of Urology, Centre de Recerca Biomèdica CELLEX, Hospital Clínic, Universitat de Barcelona, IDIBAPS, C/Casanova, 143, Barcelona, 08036, Barcelona, España

    Lourdes Mengual Ph.D.

  2. Group of Biomedical Research in Urology, Vall d’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), Barcelona, Spain

    Mireia Olivan

Authors
  1. Lourdes Mengual Ph.D.
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  2. Mireia Olivan
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Corresponding author

Correspondence to Lourdes Mengual Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Urology, Heinrich Heine University, Düsseldorf, Germany

    Wolfgang A Schulz

  2. Department of Urology, Heinrich Heine University, Düsseldorf, Germany

    Michèle J. Hoffmann

  3. Department of Urology, Heinrich Heine University, Düsseldorf, Germany

    Günter Niegisch

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Mengual, L., Olivan, M. (2018). Quantitative RNA Analysis from Urine Using Real Time PCR. In: Schulz, W., Hoffmann, M., Niegisch, G. (eds) Urothelial Carcinoma. Methods in Molecular Biology, vol 1655. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7234-0_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7234-0_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7233-3

  • Online ISBN: 978-1-4939-7234-0

  • eBook Packages: Springer Protocols

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