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The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model

  • Paula A. OliveiraEmail author
  • Cármen Vasconcelos-Nóbrega
  • Rui M. Gil da Costa
  • Regina Arantes-Rodrigues
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1655)

Abstract

Urinary bladder cancer (UBC) is a common and complex malignancy, with a multifactorial etiology, like environmental factors, such as cigarette smoking, occupational exposure, and genetic factors.

UBC exhibits considerable genotypic and phenotypic heterogeneity. Among all UBC lesions, urothelial carcinoma is the most frequently observed histological type. Despite all the developments made in urologic oncology field, therapeutic options remain inadequate. There is urgency for the identification and development of new antineoplastic drugs to replace or improve current protocols and in vivo models have been proven to be essential for this step. There are different animal models of UBC: Spontaneous and experimentally induced models (genetically engineered, transplantable-xenograft and syngeneic animals- and chemically induced models). N-butyl-N(4-hydroxybutil)nitrosamine (BBN) is the most suitable reagent to generate chemically induced in vivo models of UBC and to study bladder carcinogenesis. BBN has proven, over the years, to be very realistic and reliable. It is bladder specific, and induces high tumor incidence.

Key words

Bladder cancer Chemical carcinogenesis Arylamines Animal models 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Paula A. Oliveira
    • 1
    • 2
    Email author
  • Cármen Vasconcelos-Nóbrega
    • 3
  • Rui M. Gil da Costa
    • 1
    • 2
  • Regina Arantes-Rodrigues
    • 1
    • 2
  1. 1.Department of Veterinary SciencesUniversity of Trás-os-Montes and Alto Douro (UTAD)Vila RealPortugal
  2. 2.Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB)Vila RealPortugal
  3. 3.Centre for the Study of Education, Technologies and HealthPolythecnic Institute of ViseuViseuPortugal

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