Abstract
Here we describe a collection of methods that have been adapted to isolate and modify tumor-specific promoters (TSPs) to drive viral replication for cancer therapy and other uses. We will describe as examples the secreted protein acidic and rich in cysteine (SPARC ) and the protease-activated receptor-1 (PAR-1) promoter. We outline strategies to select appropriate TSPs using bioinformatics resources and the methods utilized in their subsequent cloning, assessment of transcriptional activity, and their use in conditionally replicative oncolytic adenoviruses.
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References
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Lopez, M.V., Cafferata, E.G., Viale, D.L., Podhajcer, O.L. (2017). Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_9
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DOI: https://doi.org/10.1007/978-1-4939-7223-4_9
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