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Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay

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Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

Abstract

Confirming the binding of a transcription factor with a particular DNA sequence may be important in characterizing interactions with a synthetic promoter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.

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References

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Authors and Affiliations

  1. Bone and Joint Research Unit, William Harvey Research Institute, Queen Mary University of London, London, EC1M 6BQ, UK

    Nasim Yousaf

  2. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, EC1M 6BQ, UK

    David Gould

Authors
  1. Nasim Yousaf
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  2. David Gould
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Corresponding author

Correspondence to David Gould .

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Editors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Yousaf, N., Gould, D. (2017). Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

  • eBook Packages: Springer Protocols

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