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Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment

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Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

Abstract

Activity of endogenous promoters can be altered by including additional responsive elements (REs). These elements can be responsive to features of the tumor environment or alternatively to signaling pathways specifically activated in cancer cells. These REs incorporated into tumor-specific promoters can improve cancer targeting, the replicative capacity, and lytic activity of conditionally replicative adenovirus. Here we outline an approach to incorporate hypoxia and inflammation REs into a specific fragment of the SPARC promoter and the steps to clone a nucleosome positioning sequence (NPS ) identified in the osteocalcin promoter that contains a Wnt RE upstream of a heterologous synthetic promoter.

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Authors and Affiliations

  1. Laboratory of Molecular and Cellular Therapy, Leloir Institute-CONICET, Av Patricias Argentinas 435, C1405BWE, Buenos Aires, Argentina

    Eduardo G. Cafferata, Maria Veronica Lopez, Felipe J. Nuñez, Maria A. R. Maenza & Osvaldo L. Podhajcer

Authors
  1. Eduardo G. Cafferata
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  2. Maria Veronica Lopez
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  3. Felipe J. Nuñez
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  4. Maria A. R. Maenza
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  5. Osvaldo L. Podhajcer
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Corresponding author

Correspondence to Eduardo G. Cafferata .

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Editors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Cafferata, E.G., Lopez, M.V., Nuñez, F.J., Maenza, M.A.R., Podhajcer, O.L. (2017). Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

  • eBook Packages: Springer Protocols

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