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A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation

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Book cover Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

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Abstract

It has been pointed out that ultrasound could be used as a controller for bioprocesses including gene expression since its energy can noninvasively reach deep in the body. Gene expression may be timely and spatially controlled by ultrasound, thus providing necessary bioactive proteins for the targeted tissue in a timely fashion. Although there are many processes involved in gene expression control, one of the most important processes is transcription, and the promoter plays an essential role in it. There are several promoters known to be activated in response to ultrasound irradiation. However, in our opinion, an artificial promoter is more suitable for clinical use. We herein describe simple methods to construct promoters that are responsive to ultrasound irradiation by randomly combining cis-elements (transcription factor binding motifs) and thereby improve its reactivity to ultrasound irradiation.

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Acknowledgement

This study was supported in part by Grants-in-Aid for Scientific Research (C) (21500403) and (18500374) and for Young Scientist (B) (20377253) and (23791746) from Japan Society for the Promotion of Science, the Research and Development Committee Program of the Japan Society of Ultrasonics in Medicine, a New Manufacture and Venture Creation Grant (Biotechnology) of Toyama New Industry Organization, a research grant of the First Bank of Toyama Foundation, and by Collaborative Research Project of the Wakasa Wan Energy Research Center.

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Authors and Affiliations

  1. Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan

    Ryohei Ogawa Ph.D. & Takashi Kondo

  2. Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

    Akihiko Watanabe & Akihiro Morii

  3. Public Health, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

    Zheng-Guo Cui

  4. School of Allied Health Sciences, Kitasato University, Sagamihara, Japan

    Go Kagiya

  5. Urology Department, Kurobe Municipal Hospital, Kurobe, Japan

    Akihiro Morii

Authors
  1. Ryohei Ogawa Ph.D.
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  2. Go Kagiya
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  3. Akihiko Watanabe
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  4. Akihiro Morii
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  5. Zheng-Guo Cui
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  6. Takashi Kondo
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Corresponding author

Correspondence to Ryohei Ogawa Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Ogawa, R., Kagiya, G., Watanabe, A., Morii, A., Cui, ZG., Kondo, T. (2017). A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

  • eBook Packages: Springer Protocols

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