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Light-Responsive Promoters

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Book cover Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

Abstract

Recent advances in the development of light-inducible transgene expression systems have overcome many inherent drawbacks of conventional chemically regulated systems. The latest generation of those light-regulated systems that are specifically responsive to different wavelengths allows spatiotemporal control of gene expression in a so far unprecedented manner.

In this chapter, we first describe the available light-inducible gene expression systems compatible with mammalian cells and explain their underlying mechanisms. Afterward, we give a detailed protocol for the implementation of a UVB light-inducible expression system in mammalian cells.

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Acknowledgment

This work was supported by the Excellence Initiative of the German Federal and State Governments (EXC-294 and GRC-4) and the European Research Council under the European Community’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement n° 259043-CompBioMat.

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Authors and Affiliations

  1. Faculty of Biology, University of Freiburg, Freiburg, Germany

    Maximilian Hörner, Konrad Müller & Wilfried Weber

  2. Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany

    Maximilian Hörner & Wilfried Weber

  3. Centre for Biological Signalling Studies (BIOSS), University of Freiburg, Freiburg, Germany

    Maximilian Hörner, Konrad Müller & Wilfried Weber

  4. Novartis Pharma AG, Biologics Technical Development and Manufacturing, Basel, Switzerland

    Konrad Müller

Authors
  1. Maximilian Hörner
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  2. Konrad Müller
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  3. Wilfried Weber
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Corresponding author

Correspondence to Wilfried Weber .

Editor information

Editors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Hörner, M., Müller, K., Weber, W. (2017). Light-Responsive Promoters. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

  • eBook Packages: Springer Protocols

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