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An Early Chick Embryo Culture Device for Extended Continuous Observation

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Book cover Avian and Reptilian Developmental Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture.

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Acknowledgment

We thank Jörg Männer and Wolfgang Seidl for fruitful discussions.

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Authors and Affiliations

  1. Anatomy and Embryology, University Medical Centre, Kreuzbergring 36, 37075, Göttingen, Germany

    Hans-Georg Sydow, Tobias Pieper, Christoph Viebahn & Nikoloz Tsikolia

Authors
  1. Hans-Georg Sydow
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  2. Tobias Pieper
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  3. Christoph Viebahn
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  4. Nikoloz Tsikolia
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Corresponding author

Correspondence to Nikoloz Tsikolia .

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Editors and Affiliations

  1. IRCMS, Kumamoto University, Kumamoto, Kumamoto, Japan

    Guojun Sheng

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Sydow, HG., Pieper, T., Viebahn, C., Tsikolia, N. (2017). An Early Chick Embryo Culture Device for Extended Continuous Observation. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

  • eBook Packages: Springer Protocols

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