Abstract
Electroporation enables delivering bionanomolecules, such as DNAs, RNAs, siRNAs, and morpholinos, into chick embryos in a spatially and temporally restricted fashion. Recent advances in electroporation techniques allowed us to deliver transgenes into the restricted area of the limb bud and to analyze the function of the enhancers in the limb field. Here, we describe the introduction of transgenes by electroporation in the limb field and its application on enhancer analysis.
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Acknowledgment
We thank Toshihiko Ogura and Jun Miyazaki for providing pCAGGS vectors, and Hajime Ogino for an EGFP reporter vector. We also thank Koh Onimaru for providing figures. This work was supported in part by a Grant-in-Aid for Scientific Research (B) (25291086) to M.T.
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Ueda, S., Suzuki, T., Tanaka, M. (2017). Transgene Introduction into the Chick Limb Bud by Electroporation. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_13
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DOI: https://doi.org/10.1007/978-1-4939-7216-6_13
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