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In Ovo Electroporation Methods in Chick Embryos

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

To elucidate a gene function, in vivo analysis is indispensable. We can carry out gain and loss of function experiment of a gene of interest by electroporation in ovo and ex ovo culture system on early-stage and advanced-stage chick embryos, respectively. In this section, we introduce in/ex ovo electroporation methods for the development of the chick central nervous system and visual system investigation.

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Correspondence to Hidekiyo Harada .

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Harada, H., Omi, M., Nakamura, H. (2017). In Ovo Electroporation Methods in Chick Embryos. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

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