Abstract
The method described here aims at the construction of a single-cell resolution gene expression atlas for an animal or tissue, combining in situ hybridization (ISH) and single-cell mRNA-sequencing (scRNAseq).
A high resolution and medium-coverage gene expression atlas of an animal or tissue of interest can be obtained by performing a series of ISH experiments, followed by a process of image registration and gene expression averaging. Using the overlapping fraction of the genes, concomitantly obtained scRNAseq data can be fitted into the spatial context of the gene expression atlas, complementing the coverage by genes.
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Achim, K., Vergara, H.M., Pettit, JB. (2018). Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas. In: Gaspar, I. (eds) RNA Detection. Methods in Molecular Biology, vol 1649. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7213-5_7
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DOI: https://doi.org/10.1007/978-1-4939-7213-5_7
Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7213-5
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