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Prion Diseases pp 197-207 | Cite as

Exosomes in Prion Diseases

  • Alexander Hartmann
  • Hermann Altmeppen
  • Susanne Krasemann
  • Markus GlatzelEmail author
Protocol
  • 374 Downloads
Part of the Neuromethods book series (NM, volume 129)

Abstract

Dementias are characterized by generation and tissue deposition of proteins altered in their secondary or tertiary structure. Prion diseases are prominent and well-studied examples of these diseases. Initiation of prion disease is associated to the conversion of the cellular prion protein (PrPC) to its pathogenic isoform (PrPSc). Spread of PrPSc throughout the central nervous system leads to disease progression and is achieved by cell-to-cell transfer, axonal or nanotube-mediated transport or exosomes.

In this chapter we describe how to isolate, purify, and quality control exosomes, and provide helpful notes for practical guidance and troubleshooting in these techniques.

Key words

Exosomes Prion protein PrP Neurotoxicity Neurodegeneration Protein aggregation Protocol 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Alexander Hartmann
    • 1
  • Hermann Altmeppen
    • 1
  • Susanne Krasemann
    • 1
  • Markus Glatzel
    • 1
    Email author
  1. 1.Institute of NeuropathologyUniversity Medical Center Hamburg-EppendorfHamburgGermany

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