Abstract
von Willebrand disease (VWD) is reportedly the most common inherited bleeding disorder and can also arise as an acquired syndrome (AVWS). These disorders develop due to defects and/or deficiency of the plasma protein von Willebrand factor (VWF). Laboratory testing for these VWF-related disorders requires assessment of both VWF level and VWF activity, the latter requiring multiple assays because of the many functions carried out by VWF to help prevent bleeding. The current paper describes several protocols for assessment of VWF activity by means of VWF ristocetin cofactor (VWF:RCo). These assays identify VWF activity by quantitative assessment of VWF protein adhesion to platelets or other particles and subsequent detection of the adhered VWF as facilitated by inclusion of ristocetin. The most commonly performed assays for VWF:RCo comprise platelet agglutination assays, latex agglutination assays, and chemiluminescent assay (CLIA), with three of these described in this chapter.
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Abbreviations
- AVWS:
-
Acquired von Willebrand syndrome
- CLIA:
-
Chemiluminescent assay
- EQA:
-
External quality assessment
- FVIII:
-
Factor VIII
- GP:
-
Glycoprotein
- HMWM:
-
High molecular weight multimers
- RLUs:
-
Relative light units
- VWF:
-
von Willebrand factor
- VWF:Ag:
-
von Willebrand factor antigen
- VWF:CB:
-
VWF collagen binding assay
- VWF:RCo:
-
VWF ristocetin cofactor assay
- VWD:
-
von Willebrand disease
References
Favaloro EJ (2011) Von Willebrand disease: local diagnosis and management of a globally distributed bleeding disorder. Semin Thromb Hemost 37:425–426
Federici AB, Budde U, Castaman G, Rand JH, Tiede A (2013) Current diagnostic and therapeutic approaches to patients with acquired von Willebrand syndrome: a 2013 update. Semin Thromb Hemost 39:191–201
Yee A, Kretz CA (2014) Von Willebrand factor: form for function. Semin Thromb Hemost 40:17–27
Favaloro EJ (2015) Recent advances in laboratory-aided diagnosis of von Willebrand disease. Expert Opin Orphan Drugs 3:975–995
Favaloro EJ, Pasalic L, Curnow J (2016) Laboratory tests used to help diagnose von Willebrand disease: an update. Pathology 48:303–318
Favaloro EJ (2014) Diagnosing von Willebrand disease: a short history of laboratory milestones and innovations, plus current status, challenges, and solutions. Semin Thromb Hemost 40:551–570
Favaloro EJ, Mohammed S, McDonald J (2010) Validation of improved performance characteristics for the automated von Willebrand factor ristocetin cofactor activity assay. J Thromb Haemost 8:2842–2844
Favaloro EJ, Mohammed S (2014) Towards improved diagnosis of von Willebrand disease: comparative evaluations of several automated von Willebrand factor antigen and activity assays. Thromb Res 134:1292–1300
Favaloro EJ, Mohammed S (2016) Evaluation of a von Willebrand factor three test panel and chemiluminescent-based assay system for identification of, and therapy monitoring in, von Willebrand disease. Thromb Res 141:202–211
Bodó I, Eikenboom J, Montgomery R, Patzke J, Schneppenheim R, Di Paola J (2015) Platelet dependent von Willebrand factor activity. Nomenclature and methodology: communication from SSC of the ISTH. J Thromb Haemost 13:1345–1350
Hillarp A, Stadler M, Haderer C, Weinberger J, Kessler CM, Romisch J (2010) Improved performance characteristics of the von Willebrand factor ristocetin cofactor activity assay using a novel automated assay protocol. J Thromb Haemost 8:2216–2223
Lippi G, Salvagno GL, Montagnana M, Lima-Oliveira G, Guidi GC, Favaloro EJ (2012) Quality standards for sample collection in coagulation testing. Semin Thromb Hemost 38:565–575
Adcock Funk DM, Lippi G, Favaloro EJ (2012) Quality Standards for sample processing, transportation, and storage in hemostasis testing. Semin Thromb Hemost 38:576–585
Clinical and Laboratory Standards Institute (2008) Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays and Molecular Hemostasis Assays; Approved Guideline, 5th edn CLSI Document edn. Clinical and Laboratory Standards Institute, Wayne, PA, pp H21–HA5
Favaloro EJ, Soltani S, McDonald J (2004) Potential laboratory misdiagnosis of haemophilia and von Willebrand disorder due to cold activation of blood samples for testing. Am J Clin Pathol 122:686–692
Böhm M, Täschner S, Kretzschmar E, Gerlach R, Favaloro EJ, Scharrer I (2006) Cold storage of citrated whole blood induces drastic time- dependent losses in factor VIII and von Willebrand factor: potential for misdiagnosis of haemophilia and von Willebrand’s disease. Blood Coagul Fibrinolysis 17:39–45
Woodhams B, Girardot O, Blanco MJ, Colesse G, Gourmelin Y (2001) Stability of coagulation proteins in frozen plasma. Blood Coagul Fibrinolysis 12:229–236
Favaloro EJ, Franchini M, Lippi G (2014) Aging hemostasis: changes to laboratory markers of hemostasis as we age. A narrative review. Semin Thromb Hemost 40:621–633
Nichols WL, Hultin MB, James AH, Manco-Johnson MJ, Montgomery RR, Ortel TL, Rick ME et al (2008) Von Willebrand disease (VWD): evidence-based diagnosis and management guidelines, the National Heart, Lung, and Blood Institute (NHLBI) expert panel report (USA). Haemophilia 14:171–232
Favaloro EJ, Soltani S, McDonald J, Grezchnik E, Easton L, Favaloro JWC (2005) Reassessment of ABO-blood group, gender and age on laboratory parameters used to diagnose von Willebrand disorder (VWD): potential influence on the diagnosis versus the potential association with risk of thrombosis. Am J Clin Pathol 124:910–917
Laffan MA, Lester W, O’Donnell JS, Will A, Tait RC, Goodeve A, Millar CM, Keeling DM (2014) The diagnosis and management of von Willebrand disease: a United Kingdom Haemophilia Centre doctors organization guideline approved by the British Committee for Standards in Haematology. Br J Haematol 167:453–465
Acknowledgments
The authors thank current laboratory staff (Jane McDonald, Ella Grezchnik, Monica Ahuja, Shabana Azimulla, and Yifang Zhang) for regular performance of the VWF testing in the Westmead Laboratory. We also thank IL Werfen for previous and ongoing assistance with CLIA-based assay evaluations, as well as for checking the latex- and the CLIA-based VWF:RCo protocols outlined in this paper.
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Mohammed, S., Favaloro, E.J. (2017). Laboratory Testing for von Willebrand Factor Ristocetin Cofactor (VWF:RCo). In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_32
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DOI: https://doi.org/10.1007/978-1-4939-7196-1_32
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