Abstract
Actvated partial thromboplastin time and its congener test Silica clotting time are used for the laboratory detection of lupus anticoagulants. Their performance consists of mixing test plasma with activators of the contact coagulation factors, phospholipids and calcium chloride, and recording the clotting time. Here we describe the principle, performance and extensive practical details of the two methods, the problems that may be encountered and how they can be identified and overcome.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tripodi A, de Groot PG, Pengo V (2011) Antiphospholipid syndrome: laboratory detection, mechanisms of action and treatment. J Intern Med 270:110–122
Pengo V, Tripodi A, Reber G et al (2009) Update of the guidelines for lupus anticoagulant detection. J Thromb Haemost 7:1737–1740
Tripodi A (2008) The history of phenotypic testing in thrombosis and hemostasis. Semin Thromb Hemost 34:585–592
Chantarangkul V, Tripodi A, Arbini A et al (1992) Silica clotting time (SCT) as a screening and confirmatory test for detection of the lupus anticoagulants. Thromb Res 67:355–365
Chantarangkul V, Tripodi A, Clerici M et al (2002) Laboratory diagnosis of lupus anticoagulants. Effect of residual platelets in plasma, assessed by Staclot-LA® and silica clotting time. Thromb Haemost 87:854–858
Tripodi A, Lippi G, Plebani M (2016) How to report results of prothrombin and activated partial thromboplastin times. Clin Chem Lab Med 54:215–222
Chantarangkul V, Biguzzi E, Asti D et al (2013) Laboratory diagnostic outcome applying detection criteria recommended by the Scientific and Standardization Committee of the ISTH on Lupus Anticoagulant. Thromb Haemost 110:46–52
Tripodi A, Padovan L, Testa S et al (2015) How the direct oral anticoagulant Apixaban affects hemostatic parameters. Results of a Multicenter Multiplatform Study. Clin Chem Lab Med 53:265–273
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Tripodi, A., Chantarangkul, V. (2017). Lupus Anticoagulant Testing: Activated Partial Thromboplastin Time (APTT) and Silica Clotting Time (SCT). In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7196-1_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7194-7
Online ISBN: 978-1-4939-7196-1
eBook Packages: Springer Protocols