Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors

  • Zhili Xu
  • Jie Tian
  • Andrew W. Harmon
  • Andrew P. Byrnes
Part of the Methods in Molecular Biology book series (MIMB, volume 1643)


Natural IgM antibodies have an innate ability to recognize many viruses and viral-based gene therapy vectors. Naive mice have natural IgM antibodies that bind to adenoviruses, and these antibodies can profoundly affect the biodistribution and efficiency of gene delivery by adenovirus type 5 vectors. Here, we present protocols for isolating IgM from mouse serum, for assaying the concentration and adenoviral reactivity of mouse IgM, and for evaluating how natural antibodies and complement can synergize to neutralize adenovirus vectors.

Key words

Natural IgM antibodies Adenovirus type 5 vectors Mouse Complement Gene delivery Virus 



This work was supported by the FDA, including funding from the CBER’s Critical Path program. We thank Mike Havert and Nirjal Bhattarai for reviewing the manuscript.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Zhili Xu
    • 1
  • Jie Tian
    • 1
  • Andrew W. Harmon
    • 1
  • Andrew P. Byrnes
    • 1
  1. 1.Division of Cellular and Gene Therapies, Center for Biologics Evaluation and ResearchFood and Drug AdministrationSilver SpringUSA

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