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Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans

  • Chunguang Wang
  • Sohvi Hörkkö
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1643)

Abstract

Natural antibodies are produced by B lymphocytes without exogenous antigenic exposure and are present at the time of birth. They usually bind to conserved epitopes on antigens of different chemical compositions. We cloned and characterized a natural mouse monoclonal IgM antibody (Aa_Mab) by selecting the binding to malondialdehyde acetaldehyde (MAA) adducts on low-density lipoprotein (LDL). The data showed that the Aa_Mab cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, an important oral pathogen in periodontitis associated with atherosclerosis. Surprisingly, the binding molecule of Aa bacteria to the Aa_Mab was Aa chaperonin 60 or HSP60, a protein that is not only responsible for maintaining cellular proteins conformation, but also functions as a potent virulence factor prompting bone resorption in periodontitis and as a putative pathogenic factor in atherosclerosis.

Key words

Natural antibody Oxidized low-density lipoprotein Malondialdehyde acetaldehyde Aggregatibacter actinomycetemcomitans Chaperonin 60 

Notes

Acknowledgment

This study was funded by Sigrid Juselius Foundation and Finnish Foundation for Cardiovascular Research.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Medical Microbiology and Immunology, Research Unit of Biomedicine, Faculty of MedicineUniversity of OuluOuluFinland
  2. 2.Medical Research Center and Nordlab OuluUniversity Hospital and University of OuluOuluFinland

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