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Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles

  • Florian Puhm
  • Christoph J. Binder
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1643)

Abstract

Natural IgM antibodies specific for oxidation-specific epitopes (OSEs) have been found to bind to circulating microvesicles (MVs), also known as microparticles. This chapter describes how endogenous natural IgM antibodies bound to circulating MV subsets can be characterized on the one hand, and how monoclonal natural IgM antibodies can be used to characterize subsets of circulating MVs on the other hand.

Key words

Natural IgM antibodies Oxidation-specific epitopes Microvesicles Microparticles Flow cytometry 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Laboratory MedicineMedical University of ViennaViennaAustria
  2. 2.Research Center for Molecular Medicine (CeMM) of the Austrian Academy of SciencesViennaAustria

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