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Methods for Posttranslational Induction of Polyreactivity of Antibodies

  • Maxime Lecerf
  • Annaelle Jarossay
  • Srinivas V. Kaveri
  • Sébastien Lacroix-Desmazes
  • Jordan D. Dimitrov
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1643)

Abstract

An antibody molecule that recognizes multiple unrelated antigens is defined as polyreactive. Polyreactivity is an intrinsic characteristic of immune repertoires. Degenerated antigen binding diversifies the repertoire of specificities, thus contributing to immune defense and immune regulation. Immune repertoire contains also a fraction of immunoglobulins, which acquire polyreactivity only following contact with various protein-destabilizing or pro-oxidative substances. Posttranslational induction of the antibody polyreactivity may have important repercussion for laboratory practice, as well as in cases of pathological conditions accompanied by liberation of large quantities of pro-oxidative substances such as heme, labile iron, or reactive oxygen species. Antibodies with induced polyreactivity have been demonstrated to exert pathogen neutralization and immune regulatory potential in inflammatory conditions, suggesting that this phenomenon may be exploited for design of therapeutic strategies. In this article, we provide description of the basic procedures for uncovering of the cryptic polyreactivity of antibodies by heme, ferrous ions, and acid pH solution.

Key words

Immunoglobulins Natural antibodies IgG Antibody polyreactivity Cryptic antigenic specificities Heme Iron ions Acidic pH 

Notes

Acknowledgments

This work was supported by INSERM (France) and by grant from Agence Nationale de la Recherche (ANR-13-JCV1-006-01).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Maxime Lecerf
    • 1
    • 2
    • 3
  • Annaelle Jarossay
    • 1
    • 2
    • 3
  • Srinivas V. Kaveri
    • 1
    • 2
    • 3
  • Sébastien Lacroix-Desmazes
    • 1
    • 2
    • 3
  • Jordan D. Dimitrov
    • 1
    • 2
    • 3
  1. 1.Sorbonne Universités, UPMC Univ Paris 06, UMR_S 1138Centre de Recherche des CordeliersParisFrance
  2. 2.INSERM, UMR_S 1138Centre de Recherche des CordeliersParisFrance
  3. 3.Université Paris Descartes, Sorbonne Paris Cité, UMR_S 1138Centre de Recherche des CordeliersParisFrance

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