Principles and Methods of Immunohistochemistry

  • José A. Ramos-VaraEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1641)


Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and the reagents used. Pretreatments such as blocking steps for endogenous activities and antigen retrieval are included. Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized.

Key words

Antigen retrieval Detection methods Fixation Immunohistochemistry Standardization Troubleshooting 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Comparative PathobiologyPurdue UniversityWest LafayetteUSA

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