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Tissue Sampling and Processing for Histopathology Evaluation

  • Mohamed SlaouiEmail author
  • Anne-Laure Bauchet
  • Laurence Fiette
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1641)

Abstract

Histological procedures aim at providing good-quality sections that can be used for a light microscopic evaluation of tissue. These are applicable to identify either spontaneous or diseases-induced changes. Routinely, tissues are fixed with neutral formalin 10%, embedded in paraffin, and manually sectioned with a microtome to obtain 4–5 μm thick paraffin sections. Dewaxed sections are then stained with HE&S (hematoxylin-eosin and saffron) or can be used for other purposes (special stains, immunohistochemistry, in situ hybridization, etc.). During this processing, many steps and procedures are critical to ensure standard and interpretable sections. This chapter provides key recommendations to efficiently achieve this objective.

Key words

Histology Embedding Sectioning Staining Histological slides Anatomic pathology Microscopy Necropsy 

References

  1. 1.
    Paget EG, Thompson R (1979) Standard operating procedures. In: Pathology. MTP Press, Lancaster, pp 134–139Google Scholar
  2. 2.
    Makki JS (2016) Diagnostic implication and clinical relevance of ancillary techniques in clinical pathology practice. Clin Med Insights Pathol 9:5–11CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Leong AS-Y (1994) Fixation and fixatives. In: Woods AE, Ellis RC (eds) Laboratory histopathology, a complete reference, vol 1. Churchill Livingstone, New York, pp 4.1-1–4.2-26Google Scholar
  4. 4.
    Ruehl-Fehlert C, Kittel B, Morawietz G, Deslex P, Keenan C, Mahrt CR, Nolte T, Robinson M, Stuart BP, Deschl U (2003) Revised guides for organ sampling and trimming in rats and mice—part 1. Exp Toxicol Pathol 55:91–106CrossRefPubMedGoogle Scholar
  5. 5.
    Kittel B, Ruehl-Fehlert C, Morawietz G, Klapwijk J, Elwell MR, Lenz B, O’Sullivan MG, Roth DR, Wadsworth PF (2004) Revised guides for organ sampling and trimming in rats and mice—part 2. Exp Toxicol Pathol 55:413–431CrossRefPubMedGoogle Scholar
  6. 6.
    Morawietz G, Ruehl-Fehlert C, Kittel B, Bube A, Keane K, Halm S, Heuser A, Hellman J (2004) Revised guides for organ sampling and trimming in rats and mice—part 3. Exp Toxicol Pathol 55:433–449CrossRefPubMedGoogle Scholar
  7. 7.
    Revised guides for organ sampling and trimming in rats and mice (2003) http://reni.item.fraunhofer.de/reni/trimming/
  8. 8.
    Soul NW (1987) Gut rolls: a better technique for GI tumor assessment. Toxicol Pathol 15:374Google Scholar
  9. 9.
    Moolenbeek C, Ruitenberg EJ (1981) The “Swiss roll”: a simple technique for histological studies of the rodent intestine. Lab Anim 15:57–59CrossRefPubMedGoogle Scholar
  10. 10.
    Piper RC (1981) Morphological evaluation of the heart in toxicologic studies. In: Balazc T (ed) Cardiac toxicology, vol 3. CRC, Boca Raton, pp 111–136Google Scholar
  11. 11.
    Latendresse JR, Warbrittion AR, Jonassen H, Creasy DM (2002) Fixation of testes and eyes using a modified Davidson’s fluid: comparison with Bouin’s fluid and conventional Davidson’s fluid. Toxicol Pathol 30:524–533CrossRefPubMedGoogle Scholar
  12. 12.
    Lanning LL, Creasy DM, Chapin RE, Mann PC, Barlow NJ, Regan KS, Goodman DG (2002) Recommended approaches for the evaluation of testicular and epididymal toxicity. Toxicol Pathol 30:507–520CrossRefPubMedGoogle Scholar
  13. 13.
    Creasy DM, Foster PMD (2002) Male reproductive system. In: Haschek WM, Rousseaux CG, Wallig MA (eds) Handbook of toxicologic pathology, vol 2. Academic, San Diego, pp 785–846CrossRefGoogle Scholar
  14. 14.
    Heindel JJ, Chapin RE (1993) Female reproductive toxicology. In: Heindel JJ, Chapin RE (eds) Methods in reproductive toxicology, vol 3B. Academic, OrlandoGoogle Scholar
  15. 15.
    McGavin MD (1991) Procedures for morphological studies of skeletal muscle, rat, mouse, and hamster. In: Jones TC, Mohr U, Hunt RD (eds) Cardiovascular and musculoskeletal systems, monographs on pathology of laboratory animals. Springer, New York, pp 101–108CrossRefGoogle Scholar
  16. 16.
  17. 17.
    Titford M, Horenstein M (2005) Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Arch Pathol Lab Med 129(4):502–506PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Mohamed Slaoui
    • 1
    Email author
  • Anne-Laure Bauchet
    • 2
  • Laurence Fiette
    • 3
  1. 1.Preclinical SafetySanofi R&DVitrysur-SeineFrance
  2. 2.Translational Medicine and Early Development, Biomarkers and Clinical BioanalysisSanofi R&DVitry-sur-SeineFrance
  3. 3.IMMRParisFrance

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