Next-Generation Sequencing to Investigate Urinary microRNAs from Macaca fascicularis (Cynomolgus Monkey)

  • Yaligara VeeranagoudaEmail author
  • Jean-François Léonard
  • Jean-Charles Gautier
  • Eric Boitier
Part of the Methods in Molecular Biology book series (MIMB, volume 1641)


Advanced sequencing technologies like next-generation sequencing (NGS) not only detect microRNAs (miRNAs) in biological samples but also facilitate de novo identification of miRNAs. Using an Ion Torrent’s Ion Proton System, here we described miRNAs sequencing of urine samples collected from Macaca fascicularis (Cynomolgus monkey) to investigate miRNAs as potential novel biomarkers of nephrotoxicity in this species. Urinary miRNA sequencing methodologies described here include (a) urinary exosomal RNA isolation, (b) sequencing library preparation, (c) sequencing template preparation, and (d) template library sequencing using Ion Proton System. The sequencing method presented in this study serves as a valuable resource in the identification of novel urinary miRNAs in M. fascicularis.

Key words

Next-Generation Sequencing microRNAs Ion Torrent Cynomolgus monkey Urine Biomarkers Kidney 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Yaligara Veeranagouda
    • 1
    Email author
  • Jean-François Léonard
    • 2
  • Jean-Charles Gautier
    • 3
  • Eric Boitier
    • 2
  1. 1.Molecular Biology & Genomics, Translational ScienceSanofi R&DChilly-MazarinFrance
  2. 2.Translational Medicine and Early Development, Biomarkers and Clinical Bioanalyses—GenomicsSanofi R&DVitry-sur-SeineFrance
  3. 3.Preclinical SafetySanofi, R&DVitry-sur-SeineFrance

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