Abstract
The frog embryo teratogenesis assay Xenopus (FETAX) test is a development toxicity screening test. Due to the small amount of compound needed and the capability to study organogenesis in a short period of time (96 h), FETAX test constitutes an efficient development toxicity alert test when performed early in drug safety development. The test is conducted on fertilized Xenopus laevis mid-blastula-stage eggs over the organogenesis period. Compound teratogenic potential is determined after analysis of the mortality and malformation observations on larvae. In parallel, FETAX test provides also information concerning embryotoxic effect based on larva length.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
ASTM (1998) Standard guide for conducting the frog embryo teratogenesis assay – Xenopus. Designation E 1439-98, Annual book of ASTM standards, vol 11.5. American Society for Testing and Materials, Philadelphia, pp 825–836
Bantle JA, Finch RA, Burton DT, Fort DJ, Dawson DA, Linder G, Rayburn JR, Hull M, Kumsher-King M, Gaudet-Hull AM, Turley SD (1996) FETAX interlaboratory validation study: Phase III – Part 1 testing. J Appl Toxicol 16(6):517–528
Leconte I, Mouche I (2013) Frog embryo teratogenesis assay on Xenopus and predictivity compared with in vivo mammalian studies. Methods Mol Biol 947:403–421
ASTM (2012) Standard guide for conducting the frog embryo teratogenesis assay – Xenopus Designation E 1439-12, ASTM book of standards, vol 11.6. American Society for Testing and Materials, Philadelphia
Sipe CW, Saha MS (2007) The use of microarray technology in non mammalian vertebrate systems. In: Rampal JB (ed) Microarrays: volume II: applications and data analysis, Methods in Molecular Biology, vol 382. Humana, Totowa, NJ, pp 1–16
Vallée M, Aiba K, Piao Y, Palin MF, Ko MS, Sirard MA (2008) Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species. Reproduction 135(4):439–448
Bantle JA, Dumont JN, Finch RA, Linder G, Fort DJ (1998) Atlas of abnormalities, a guide for the performance of FETAX, 2nd edn. Oklahoma State University, Stillwater, OK
Fort DJ, Stover EL, Bantle JA, Rayburn JR, Hull MA, Finch RA, Burton DT, Turley SD, Dawson DA, Linder G, Buchwalter D, Dumont JN, Kumsher-King M, Gaudet-Hull AM (1998) Phase III interlaboratory study of FETAX, Part 2: interlaboratory validation of an exogenous metabolic activation system for frog embryo teratogenesis assay-Xenopus (FETAX). Drug Chem Toxicol 21(1):1–14
Bantle JA, Finch RA, Fort DJ, Stover EL, Hull M, Kumsher-King M, Gaudet-Hull AM (1999) Phase III interlaboratory study of FETAX, Part 3: FETAX validation using 12 compounds with and without an exogenous metabolic activation system. J Appl Toxicol 19:447–472
Fort DJ, Rogers RL, Stover EL, Finch RA (2001) Optimization of an exogenous metabolic activation system for FETAX. I. Post-isolation rat liver microsome mixtures. Drug Chem Toxicol 24(2):103–115
Fort DJ, Rogers RL, Paul RR, Stover EL, Finch RA (2001) Optimization of an exogenous metabolic activation system for FETAX. II. Preliminary evaluation. Drug Chem Toxicol 24(2):117–127
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Mouche, I., Malésic, L., Gillardeaux, O. (2017). FETAX Assay for Evaluation of Developmental Toxicity. In: Gautier, JC. (eds) Drug Safety Evaluation. Methods in Molecular Biology, vol 1641. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7172-5_17
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7172-5_17
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7170-1
Online ISBN: 978-1-4939-7172-5
eBook Packages: Springer Protocols