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NMR and MS Methods for Metabolomics

  • Alexander Amberg
  • Björn RiefkeEmail author
  • Götz Schlotterbeck
  • Alfred Ross
  • Hans Senn
  • Frank Dieterle
  • Matthias Keck
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1641)

Abstract

Metabolomics, also often referred as “metabolic profiling,” is the systematic profiling of metabolites in biofluids or tissues of organisms and their temporal changes. In the last decade, metabolomics has become more and more popular in drug development, molecular medicine, and other biotechnology fields, since it profiles directly the phenotype and changes thereof in contrast to other “-omics” technologies. The increasing popularity of metabolomics has been possible only due to the enormous development in the technology and bioinformatics fields. In particular, the analytical technologies supporting metabolomics, i.e., NMR, UPLC-MS, and GC-MS, have evolved into sensitive and highly reproducible platforms allowing the determination of hundreds of metabolites in parallel. This chapter describes the best practices of metabolomics as seen today. All important steps of metabolic profiling in drug development and molecular medicine are described in great detail, starting from sample preparation to determining the measurement details of all analytical platforms, and finally to discussing the corresponding specific steps of data analysis.

Key words

Metabolomics Metabolic profiling NMR UPLC-MS GC-MS 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Alexander Amberg
    • 1
  • Björn Riefke
    • 2
    Email author
  • Götz Schlotterbeck
    • 3
  • Alfred Ross
    • 4
  • Hans Senn
    • 5
  • Frank Dieterle
    • 6
  • Matthias Keck
    • 7
  1. 1.Preclinical SafetySanofi R&DHattersheimGermany
  2. 2.Investigational Toxicology, Metabolic Profiling and Clinical PathologyBayer Pharma AGBerlinGermany
  3. 3.School of Life Sciences, Institute for Chemistry and BioanalyticsUniversity of Applied Sciences, Northwestern SwitzerlandMuttenzSwitzerland
  4. 4.Roche Pharmaceutical Research and Early Development, Pharmaceutical SciencesRoche Innovation Center Basel, F. Hoffmann-La Roche LtdBaselSwitzerland
  5. 5.Heythrop College UCL, Kensington SquareLondon W85HNUK
  6. 6.New Products and MedicalNear Patient Testing, NovartisBaselSwitzerland
  7. 7.Analytical Development 1Bayer Pharma AGWupperalGermany

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