Impedance Measurement in Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Impedance-based measurement is a useful tool for assessing the contractility of plated cardiomyocytes in the context of early preclinical cardiosafety assessment. Induced pluripotent stem cell-derived cardiomyocytes (iPSCs) can be used for this purpose as these cells display similar electrochemical properties to primary cardiomyocytes and beat reliably and in synchronicity in culture. Here we describe a method for measuring the contractility of iPSCs using the xCELLigence RTCA impedance measurement system.
Key wordsiPSC cardiomyocytes iCells cardiomyocytes Contractility measurement Impedance Cardiosafety
- 3.Brown AM (2005) HERG block, QT liability and sudden cardiac death. Novartis Found Symp 266:118–131; discussion 131–135, 155–158Google Scholar
- 6.Denning C, Anderson D (2008) Cardiomyocytes from human embryonic stem cells as predictors of cardiotoxicity. Drug Discov Today: Ther Strateg 5(4):223–232Google Scholar
- 7.Ma J, Guo L, Fiene SJ, Anson BD, Thomson JA, Kamp TJ, Kolaja KL, Swanson BJ, January CT (2011) High purity human-induced pluripotent stem cell-derived cardiomyocytes: electrophysiological properties of action potentials and ionic currents. Am J Physiol Heart Circ Physiol 301(5):H2006–H2017CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE (2014) Automated video-based analysis of contractility and calcium flux in human-induced pluripotent stem cell-derived cardiomyocytes cultured over different spatial scales. Tissue Eng Part C Methods 21(5):467–479CrossRefGoogle Scholar
- 14.Abassi YA, Xi B, Li N, Ouyang W, Seiler A, Watzele M, Kettenhofen R, Bohlen H, Ehlich A, Kolossov E, Wang X, Xu X (2012) Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment. Br J Pharmacol 165(5):1424–1441CrossRefPubMedPubMedCentralGoogle Scholar