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Site-Specific Recombinases pp 325–339Cite as

Production of Minicircle DNA Vectors Using Site-Specific Recombinases

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1642))

Abstract

Minicircle DNA vectors are plasmid derivatives free of bacterial elements. These vectors are mostly provided from common plasmids via recombination by site-specific recombinases in E. coli. Absence of bacterial backbone in minicircle vectors results in high-level and persistent expression of transgene in comparison with conventional plasmids and provides promising vehicles for gene therapy and vaccination. Here we describe the production of replicative minicircle DNA vectors using the PBAD/araC system expressing ΦC31 integrase in E. coli.

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Authors and Affiliations

  1. Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

    Nafiseh Sanei Ata-abadi, Naeimeh Rezaei, Kianoush Dormiani & Mohammad Hossein Nasr-Esfahani

  2. Faculty of Biological Sciences, Department of Molecular Genetics, Tarbiat Modares University, Tehran, Iran

    Nafiseh Sanei Ata-abadi

Authors
  1. Nafiseh Sanei Ata-abadi
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  2. Naeimeh Rezaei
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  3. Kianoush Dormiani
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  4. Mohammad Hossein Nasr-Esfahani
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Corresponding author

Correspondence to Nafiseh Sanei Ata-abadi .

Editor information

Editors and Affiliations

  1. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA

    Nikolai Eroshenko

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Ata-abadi, N.S., Rezaei, N., Dormiani, K., Nasr-Esfahani, M.H. (2017). Production of Minicircle DNA Vectors Using Site-Specific Recombinases. In: Eroshenko, N. (eds) Site-Specific Recombinases. Methods in Molecular Biology, vol 1642. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7169-5_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7169-5_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7167-1

  • Online ISBN: 978-1-4939-7169-5

  • eBook Packages: Springer Protocols

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