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Establishment of an In Vivo ARGONAUTE Reporter System in Plants

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Plant Argonaute Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1640))

Abstract

RNA silencing is not only an evolutionarily conserved gene regulatory mechanism, but in plants also serves as the basis for robust adaptive antiviral immune responses. ARGONAUTE (AGO) proteins form the catalytic cores of the RNA-guided ribonuclease complexes, which play a central role in RNA silencing. Here we describe an in vivo assay system for analyzing the activities of AGO proteins in the virological model plant Nicotiana benthamiana .

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Acknowledgments

This work was supported by the Hungarian Scientific Research Fund (NK105850).

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Authors and Affiliations

  1. Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, Szent Györgyi Albert u. 4., Gödöllő, 2100, Hungary

    Károly Fátyol & József Burgyán

Authors
  1. Károly Fátyol
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  2. József Burgyán
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Corresponding author

Correspondence to Károly Fátyol .

Editor information

Editors and Affiliations

  1. Instituto de Biología Molecular y Celular de Plantas, (Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia), Valencia, Spain

    Alberto Carbonell

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Fátyol, K., Burgyán, J. (2017). Establishment of an In Vivo ARGONAUTE Reporter System in Plants. In: Carbonell, A. (eds) Plant Argonaute Proteins. Methods in Molecular Biology, vol 1640. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7165-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7165-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7164-0

  • Online ISBN: 978-1-4939-7165-7

  • eBook Packages: Springer Protocols

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