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Cloning and Characterization of Argonaute Genes in Tomato

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Plant Argonaute Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1640))

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Abstract

Argonaute (AGO) proteins are core elements in plant posttranscriptional RNA silencing pathways. The identification and functional characterization of tomato (Solanum lycopersicum) AGOs will help to better understand RNA silencing-based posttranscriptional regulation in fleshy fruits. Here we describe how to identify and clone SlAGO genes, as well as the methodology for their functional characterization.

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Acknowledgments

This work is supported by the National Research and Development Program (2016YFD0400101) and the National Natural Science Foundation of China (31501770).

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Authors and Affiliations

  1. School of Life Sciences, Chongqing University, No 702 Room, Campus D, Chongqing, 401331, China

    Zhiqiang Xian, Fang Yan & Zhengguo Li

Authors
  1. Zhiqiang Xian
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  2. Fang Yan
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  3. Zhengguo Li
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Corresponding author

Correspondence to Zhengguo Li .

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Editors and Affiliations

  1. Instituto de Biología Molecular y Celular de Plantas, (Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia), Valencia, Spain

    Alberto Carbonell

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Xian, Z., Yan, F., Li, Z. (2017). Cloning and Characterization of Argonaute Genes in Tomato. In: Carbonell, A. (eds) Plant Argonaute Proteins. Methods in Molecular Biology, vol 1640. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7165-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7165-7_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7164-0

  • Online ISBN: 978-1-4939-7165-7

  • eBook Packages: Springer Protocols

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