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Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair

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Plant Argonaute Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1640))

Abstract

Plant ARGONAUTE (AGO) proteins regulate a wide range of cellular and developmental functions. Recent findings highlight their role during homologous recombination, a basic mechanism to repair double-strand DNA lesions (in somatic cells) and programmed DNA breaks (in meiocytes). This chapter contains an exhaustive description of procedures applied to analyze meiotic chromosome behavior (cytogenetic techniques) and DNA repair capacity (genotoxicity assays) in AGO-deficient Arabidopsis thaliana mutants.

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Acknowledgments

MMG and MP acknowledge the support of the European Union by the advanced grant Marie Curie Initial Training Network (ITN) “COMREC” (Grant agreement number: 606956). In addition, the work in MP laboratory is supported by a grant from the Ministerio de Economía y Competitividad of Spain (AGL2015–67349–P). The authors thank Juan Luis Santos and Eugenio Sánchez–Morán for helpful discussions about the importance of accurate chiasma quantification in the analysis of meiotic mutants, and Alexander Knoll and Holger Puchta for sharing protocols related to sensitivity assays using genotoxic agents.

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Authors and Affiliations

  1. School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK

    Marina Martinez-Garcia

  2. Departamento de Genética, Facultad de Biología, Universidad Complutense de Madrid, C/Jose Antonio Novais 12, Madrid, 28040, Spain

    Mónica Pradillo

Authors
  1. Marina Martinez-Garcia
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  2. Mónica Pradillo
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Corresponding author

Correspondence to Mónica Pradillo .

Editor information

Editors and Affiliations

  1. Instituto de Biología Molecular y Celular de Plantas, (Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia), Valencia, Spain

    Alberto Carbonell

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Martinez-Garcia, M., Pradillo, M. (2017). Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair. In: Carbonell, A. (eds) Plant Argonaute Proteins. Methods in Molecular Biology, vol 1640. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7165-7_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7165-7_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7164-0

  • Online ISBN: 978-1-4939-7165-7

  • eBook Packages: Springer Protocols

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