Immunohistochemistry Staining for Human Alpha-1 Antitrypsin
Immunohistochemistry (IHC) is a powerful immunology-based method that is used to study the location of proteins in cells and tissues. There have been numerous advancements in IHC technology that continually increase the sensitivity and specificity through which this method can be used to generate new discoveries. Similarly, Alpha-1 Antitrypsin (AAT) IHC can be used to study AAT protein expression within the human liver or exogenous AAT that is delivered through gene therapy. Here, we describe a highly sensitive method to detect the AAT antigen in formalin-fixed paraffin-embedded human or mouse tissues.
Key wordsAlpha-1 antitrypsin Alpha-1 antitrypsin deficiency Protein localization Immunohistochemistry Immunoperoxidase
This work was supported by a grant from 5P01DK058327 (Dr. Barry Byrne, PI).
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