Immunohistochemistry Staining for Human Alpha-1 Antitrypsin

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1639)

Abstract

Immunohistochemistry (IHC) is a powerful immunology-based method that is used to study the location of proteins in cells and tissues. There have been numerous advancements in IHC technology that continually increase the sensitivity and specificity through which this method can be used to generate new discoveries. Similarly, Alpha-1 Antitrypsin (AAT) IHC can be used to study AAT protein expression within the human liver or exogenous AAT that is delivered through gene therapy. Here, we describe a highly sensitive method to detect the AAT antigen in formalin-fixed paraffin-embedded human or mouse tissues.

Key words

Alpha-1 antitrypsin Alpha-1 antitrypsin deficiency Protein localization Immunohistochemistry Immunoperoxidase 

Notes

Acknowledgment

This work was supported by a grant from 5P01DK058327 (Dr. Barry Byrne, PI).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Pathology, Immunology, and Laboratory Medicine, College of MedicineUniversity of FloridaGainesvilleUSA

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