Immunohistochemistry Staining for Human Alpha-1 Antitrypsin

  • Dongtao A. Fu
  • Martha Campbell-Thompson
Part of the Methods in Molecular Biology book series (MIMB, volume 1639)


Immunohistochemistry (IHC) is a powerful immunology-based method that is used to study the location of proteins in cells and tissues. There have been numerous advancements in IHC technology that continually increase the sensitivity and specificity through which this method can be used to generate new discoveries. Similarly, Alpha-1 Antitrypsin (AAT) IHC can be used to study AAT protein expression within the human liver or exogenous AAT that is delivered through gene therapy. Here, we describe a highly sensitive method to detect the AAT antigen in formalin-fixed paraffin-embedded human or mouse tissues.

Key words

Alpha-1 antitrypsin Alpha-1 antitrypsin deficiency Protein localization Immunohistochemistry Immunoperoxidase 



This work was supported by a grant from 5P01DK058327 (Dr. Barry Byrne, PI).


  1. 1.
    Wood AM, Stockley RA (2007) Alpha one antitrypsin deficiency: from gene to treatment. Respiration 74:481–492CrossRefPubMedGoogle Scholar
  2. 2.
    de Serres F, Blanco I (2014) Role of alpha-1 antitrypsin in human health and disease. J Intern Med 276:311–335CrossRefPubMedGoogle Scholar
  3. 3.
    Lomas DA, Evans DL, Finch JT, Carrell RW (1992) The mechanism of Z alpha 1-antitrypsin accumulation in the liver. Nature 357:605–607CrossRefPubMedGoogle Scholar
  4. 4.
    Teckman JH, Mangalat N (2015) Alpha-1 antitrypsin and liver disease: mechanisms of injury and novel interventions. Expert Rev Gastroenterol Hepatol 9:261–268CrossRefPubMedGoogle Scholar
  5. 5.
    Brebner JA, Stockley RA (2013) Recent advances in α-1-antitrypsin deficiency-related lung disease. Expert Rev Respir Med 7:213–229. quiz 230CrossRefPubMedGoogle Scholar
  6. 6.
    Callea F, Brisigotti M, Faa G, Lucini L, Eriksson S (1991) Identification of PiZ gene products in liver tissue by a monoclonal antibody specific for the Z mutant of alpha 1-antitrypsin. J Hepatol 12:372–376CrossRefPubMedGoogle Scholar
  7. 7.
    Janciauskiene S, Dominaitiene R, Sternby NH, Piitulainen E, Eriksson S (2002) Detection of circulating and endothelial cell polymers of Z and wild type alpha 1-antitrypsin by a monoclonal antibody. J Biol Chem 277:26540–26546CrossRefPubMedGoogle Scholar
  8. 8.
    Miranda E, Pérez J, Ekeowa UI, Hadzic N, Kalsheker N, Gooptu B, Portmann B, Belorgey D, Hill M, Chambers S, Teckman J, Alexander GJ, Marciniak SJ, Lomas DA (2010) A novel monoclonal antibody to characterize pathogenic polymers in liver disease associated with alpha1-antitrypsin deficiency. Hepatology 52:1078–1088CrossRefPubMedGoogle Scholar
  9. 9.
    Mueller C, Chulay JD, Trapnell BC, Humphries M, Carey B, Sandhaus RA, McElvaney NG, Messina L, Tang Q, Rouhani FN, Campbell-Thompson M, AD F, Yachnis A, Knop DR, Ye GJ, Brantly M, Calcedo R, Somanathan S, Richman LP, Vonderheide RH, Hulme MA, Brusko TM, Wilson JM, Flotte TR (2013) Human Treg responses allow sustained recombinant adeno-associated virus-mediated transgene expression. J Clin Invest 123:5310–5318CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Pathology, Immunology, and Laboratory Medicine, College of MedicineUniversity of FloridaGainesvilleUSA

Personalised recommendations