Abstract
This protocol describes the analysis of date palm proteome under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. Three-month-old date palm seedlings are subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for 1 month prior to leaf sample analysis. Differential in gel electrophoresis (DIGE) analysis of protein extracts identifies the sensitive proteins that respond to abiotic stress. Mass spectrometric analysis identifies the significantly changed proteins under both salt and drought stress. This chapter provides techniques for analyzing the proteome of date palm under salinity and drought stress.
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El Rabey, H.A. (2017). Proteome of Abiotic Stress Tolerance in Date Palm. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_26
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DOI: https://doi.org/10.1007/978-1-4939-7159-6_26
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7158-9
Online ISBN: 978-1-4939-7159-6
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