Abstract
Shoot-tip explants obtained from offshoots of adult date palms are an excellent source for callus induction and subsequent somatic embryogenesis. In this protocol, the shoot-tip explants are transferred sequentially to a series of media containing gradually reduced concentrations of plant growth hormones: (a) 10 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 3 mg/L 2-isopentenyl adenine (2iP), (b) 7 mg/L 2,4-D and 1 mg/L 2iP, (c) 5 mg/L 2,4-D and 1 mg/L 2iP, and (d) 3 mg/L 2,4-D and 1 mg/L 2iP. Embryogenic callus differentiates into somatic embryos upon transfer to MS medium containing 0.5 mg/L abscisic acid (ABA) and 0.1 mg/L naphthalene acetic acid (NAA). Well-matured somatic embryos germinate on a medium containing 0.1 mg/L NAA. Repeated, multiple, and secondary somatic embryos are induced to produce normal well-developed somatic embryos upon transfer to MS medium containing 0.1 mg/L NAA and 0.05 mg/L benzyladenine (BA). This protocol is potentially applicable for commercial micropropagation of date palm.
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Acknowledgment
The author expresses sincere thanks and appreciation to agronomists Ahmed Hassan El-Tanboly and Mahmoud Ali Hamza for their vital help to achieve this work.
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Zayed, Z.E. (2017). Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2,4-D Concentration. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_7
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DOI: https://doi.org/10.1007/978-1-4939-7156-5_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7155-8
Online ISBN: 978-1-4939-7156-5
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