Abstract
Magnetic resonance spectroscopy (MRS) is an analytical technique that has been extensively used to examine reprogrammed metabolism and treatment response in cancer cells and solid tumors both in vivo and ex vivo. High-resolution MRS (HR-MRS) is one of the best methods for metabolic profiling, as it is highly quantitative, robust, and reproducible. The protocols for dual-phase extraction of cancer cells and tumors and sample preparations for high-resolution 1H and 31P HR-MRS analysis are described here. Descriptions of spectra acquisition and analysis are also included in this chapter.
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Acknowledgments
This work is support by funding received from the CR-UK Cancer Imaging Centre in association with the MRC and Department of Health (England) grant C1060/A10334, C1060/A16464, NHS funding to the NIHR Biomedical Research Centre.
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Chung, YL. (2017). Magnetic Resonance Spectroscopy (MRS)-Based Methods for Examining Cancer Metabolism in Response to Oncogenic Kinase Drug Treatment. In: Tan, AC., Huang, P. (eds) Kinase Signaling Networks. Methods in Molecular Biology, vol 1636. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7154-1_25
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DOI: https://doi.org/10.1007/978-1-4939-7154-1_25
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7154-1
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