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Membrane Protein Structure and Function Characterization pp 195–203Cite as

Secondary Structure Determination by Means of ATR-FTIR Spectroscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1635))

Abstract

Specialized infrared spectroscopic techniques have been developed that allow studying the secondary structure of membrane proteins and the influence of crucial parameters like lipid content and detergent. Here, we focus on an ATR-FTIR spectroscopic study of Af-Amt1 and the influence of LDAO/glycerol on its structural integrity. Our results clearly indicate that infrared spectroscopy can be used to identify the adapted sample conditions.

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Authors and Affiliations

  1. Laboratoire de Bioelectrochimie et Spectroscopie, UMR 7140, Chimie de la Matière Complexe, Université de Strasbourg, Strasbourg, France

    Batoul Srour & Petra Hellwig

  2. Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104, Freiburg, Germany

    Stefan Bruechert

  3. BIOSS Centre for Biological Signalling Studies, Schänzlestr. 1, 79104, Freiburg, Germany

    Susana L. A. Andrade

  4. Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104, Freiburg, Germany

    Susana L. A. Andrade

Authors
  1. Batoul Srour
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  2. Stefan Bruechert
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  3. Susana L. A. Andrade
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  4. Petra Hellwig
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Corresponding author

Correspondence to Petra Hellwig .

Editor information

Editors and Affiliations

  1. Département de Chimie, CNRS UMR 7203 LBM, Sorbonne Universités—UPMC Univ Paris 06, École Normale Supérieure—PSL Research University, Paris, France

    Jean-Jacques Lacapere

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Srour, B., Bruechert, S., Andrade, S.L.A., Hellwig, P. (2017). Secondary Structure Determination by Means of ATR-FTIR Spectroscopy. In: Lacapere, JJ. (eds) Membrane Protein Structure and Function Characterization. Methods in Molecular Biology, vol 1635. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7151-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7151-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7149-7

  • Online ISBN: 978-1-4939-7151-0

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