Abstract
Enumeration of circulating tumor cells (CTCs) can provide valuable prognostic information to guide cancer treatment as well as help monitor disease progression. Analysis of these rare malignant cells has the potential to further our understanding of cancer metastasis by gaining insights into CTC characteristics and properties. Microfluidics presents a unique platform to isolate and study CTCs. In this chapter, we describe the detailed procedures for the fabrication and use of a microfluidic device to detect CTCs from the blood of pancreatic cancer patients.
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Acknowledgments
We acknowledge financial support from the National Cancer Institute (NCI-K25CA149080) and the University of Florida Health Cancer Center (UFHCC) for Pilot Project Award, and the University of Florida Division of Sponsored Research for Preparatory Grant and Opportunity Fund. Jose Varillas is supported in part by the NIH TL1 grant under the University of Florida Clinical and Translational Science Awards (TL1TR001428 and UL1TR001427) and the Robert C. Pittman fellowship of the Nanoscience Institute for Medical and Engineering Technology (NIMET).
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Varillas, J.I., Chen, K., Zhang, J., George, T.J., Hugh Fan, Z. (2017). A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples. In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_3
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DOI: https://doi.org/10.1007/978-1-4939-7144-2_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7143-5
Online ISBN: 978-1-4939-7144-2
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