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Immunophenotyping by Multiparameter Flow Cytometry

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Acute Myeloid Leukemia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1633))

Abstract

Multiparameter flow cytometry has become an indispensable tool for the diagnosis and classification of acute myeloid leukemia (AML). The basic method relies on the unique ability to detect immunophenotypic abnormalities on discrete subpopulations. The primary roles in the initial assessment of AML are to determine the immaturity of the leukemic population, define the lineage and the immunophenotypic aberrancies in blasts, and identify characteristic immunophenotypic features to predict important recurrent cytogenetic and genetic abnormalities and prognosis. The established immunophenotypic profile, a baseline “fingerprint,” is used for follow-up assessment of residual disease. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes our strategy for data analysis supplemented with illustrative case examples.

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Acknowledgments

Dr. Franklin Fuda is gratefully acknowledged for critical reading and commenting the content of this chapter. The authors also thank the medical technologists in the Clinical Flow Cytometry laboratory at the University of Texas Southwestern Medical Center for their technical assistance.

Author information

Authors and Affiliations

  1. Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA

    Weina Chen M.D., Ph.D. & Hung S. Luu

  2. Department of Pathology, Children’s Medical Center at Dallas, Univeristy of Texas Southwestern Medical Center, Dallas, TX, USA

    Hung S. Luu

Authors
  1. Weina Chen M.D., Ph.D.
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  2. Hung S. Luu
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Corresponding author

Correspondence to Weina Chen M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Sidney Kimmel Cancer Center, Philadelphia, Pennsylvania, USA

    Paolo Fortina

  2. Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Eric Londin

  3. Department of Pathology, UT Southwestern Medical Center and Children’s Medical Center, Dallas, Texas, USA

    Jason Y. Park

  4. Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA

    Larry J. Kricka

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Chen, W., Luu, H.S. (2017). Immunophenotyping by Multiparameter Flow Cytometry. In: Fortina, P., Londin, E., Park, J., Kricka, L. (eds) Acute Myeloid Leukemia. Methods in Molecular Biology, vol 1633. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7142-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7142-8_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7140-4

  • Online ISBN: 978-1-4939-7142-8

  • eBook Packages: Springer Protocols

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